Are other fluorescent tags used instead of ethidium bromide safer?
نویسندگان
چکیده
منابع مشابه
Mechanism of Ethidium Bromide Fluorescence Enhancement on
The mechanism of the enhancement of the fluorescence of ethidium bromide on binding to double helical RNA and DNA has been investigated. From an examination of the effect of different solvents on the fluorescence lifetime, quenching of fluorescence by proton acceptors, and the substantial lengthening of lifetime observed upon deuteration of the amino protons, regardless of the medium, we conclu...
متن کاملComparison of various ultraviolet sources for fluorescent detection of ethidium bromide-DNA complexes in polyacrylamide gels.
Ultraviolet sources with output wavelengths or 254, 300, and 366 nm were compared for detection of ethidium bromide-DNA complexes in acrylamide gels. The 254and 300-nni sources were both much more sensitive than the 366-nni source. The 254-nni .source produced a great deal of photodamage, photonickingand photodimerization, and photobleaching, while the longer wavelength sources cause little dam...
متن کاملEthidium bromide-resistant mutant of Bacillus subtilis.
An ethidium bromide-resistant mutant (EB8) derived from a Marburg strain of Bacillus subtilis was found to be conditionally resistant to 10 mug of ethidium bromide per ml. Expression of resistance is complete only during vegetative growth at incubation temperatures above 30 C in complex medium or minimal medium supplemented with Casamino Acids. Strain EB8 is cross-resistant to acriflavine and p...
متن کاملA continuous kinetic assay for RNA-cleaving deoxyribozymes, exploiting ethidium bromide as an extrinsic fluorescent probe.
We describe a rapid and inexpensive method to monitor the kinetics of small RNA-cleaving deoxyribozymes, based on the exogenous fluorophore ethidium bromide. Ethidium binds preferentially to double-stranded nucleic acids, and its fluorescence emission increases dramatically upon intercalation. Thus, ethidium can be used in single-turnover experiments to measure both annealing of the deoxyribozy...
متن کاملEthidium bromide as a cooperative effector of a DNA structure.
A salt-induced cooperative conformational transition of a synthetic DNA, poly(dG-dC), is reversed by addition of ethidium bromide. Binding of the dye at high salt concentrations is highly cooperative. Circular dichroism spectra of the complex and the kinetic data support a model for this cooperative binding that is formally equivalent to the "allosteric" one proposed for oligomeric proteins by ...
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ژورنال
عنوان ژورنال: DARU Journal of Pharmaceutical Sciences
سال: 2013
ISSN: 2008-2231
DOI: 10.1186/2008-2231-21-71